Opening book details…
Can I read New Ways in Bioanalysis—one-way Optical Sensor Chip for Environmental Analysis on EtoBox?
New Ways in Bioanalysis—one-way Optical Sensor Chip for Environmental Analysis by Markus Meusel; Dieter Trau; Andreas Katerkamp; Frank Meier; Rainer Polzius; Karl Cammann is a Engineering article available to read on EtoBox.
What is New Ways in Bioanalysis—one-way Optical Sensor Chip for Environmental Analysis about?
An optical immunosensor system consisting of a disposable low-cost sensor chip including a fluidic system and a base unit for optical readout was developed. Near infrared (NIR)-fluorescence markers (Cy5) were excited by an evanescent wave generated on the surface of the sensor chip. The combination of both fluorescence measurements and evanescent wave excitation provides extremely sensitive detection and avoids any washing or separation steps. To demonstrate the feasibility of the system for environmental control assays for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) were developed. Two different assay formats were applied to determine 2,4-D in the relevant concentration range. Due to the assay formats chosen a direct proportional relationship between analyte concentration and signal intensity was achieved. Within an assay time of 15 min only, the analyte 2,4-D could be determined in a linear concentration range covering three orders of magnitude.
Who reads New Ways in Bioanalysis—one-way Optical Sensor Chip for Environmental Analysis?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Markus Meusel; Dieter Trau; Andreas Katerkamp; Frank Meier; Rainer Polzius; Karl Cammann
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0925-4005)
- Published
- 1998
- Language
- EN
- Field
- Engineering (Physical Sciences)