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Amperometric Detection of Reducing Carbohydrates in High- Performance Liquid Chromatography Using an Amino-bonded Column and Acetonitrile-water as the Eluent by Noriyuki Watanabe is a scholarly article available to read on EtoBox.

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Amperometric detection of reducing carbohydrates using copper bis(phenanthroline) as the mediator in the post-column reaction was applied to the partition mode of high-performance liquid chromatography using an amino-bonded column with acetonitrile-water as the eluent. The proposed method appears to be the most sensitive means of detection in the partition separation of carbohydrates. Glucose can be determined at levels down to 5 pmol(1 ng injection). Excellent selectivity was demonstrated in examples of chromatograms obtained with fruit juices and human urine.

Author

Noriyuki Watanabe

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1985

Language

EN