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Contribution of IL-33/ILC2-mediated Th2 cytokines during the progression of minimal change disease by Cui Liu; Li Liu; Yanping Huang; Ruiming Shi; Yue Wu; Intan Hakimah Binti Ismail is a Immunology and Microbiology article available to read on EtoBox.

What is Contribution of IL-33/ILC2-mediated Th2 cytokines during the progression of minimal change disease about?

Minimal change disease (MCD) is a common type of nephrotic syndrome with high recurrence rate. This study aims to explore the impacts of interleukin (IL)–33 in MCD and to discuss its potential mechanism. In adriamycin (ADM) and puromycin aminonucleoside (PAN)-induced MCD rat model, IL-33 was used for treatment. H&E staining was applied for detecting histological changes. Critical proteins were examined by western blot. Corresponding commercial kits tested oxidative stress- and inflammation-related factors. Cell apoptosis was measured by TUNEL assay. ADM-induced podocyte injury model was establish to mimic MCD in vitro. Cell proliferation and apoptosis were detected by CCK-8 and TUNEL assays. Finally, podocyte was stimulated by innate lymphoid type-2 cells-secreted Th2 cytokines (ILC2s: IL-13 and IL-5 respectively), with or without incubation with M1 macrophage medium to further explore the immune-regulation of ILC2s behind the inflammatory environment of MCD. It was found that PAN-induced kidney jury, inflammation, oxidative stress and apoptosis were severer than ADM, and IL-33 treatment significantly alleviated the above injuries in PAN and ADM-induced MCD rat model. Moreover, IL-

Who reads Contribution of IL-33/ILC2-mediated Th2 cytokines during the progression of minimal change disease?

Author

Cui Liu; Li Liu; Yanping Huang; Ruiming Shi; Yue Wu; Intan Hakimah Binti Ismail

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Immunology and Microbiology (Life Sciences)