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This document summarizes a study on overcoming browning in ulin (Eusideroxylon zwageri) explants for regeneration initiation through in vitro culture. The study had two stages: stage 1 involved soaking explants for 24 hours in 50% MS liquid media at pH 4, which overcame browning with 100% success. Stage 2 involved culturing explants on solid MS media with 1.0 mg/L BAP supplemented with vitamin C (1 mg/L ascorbic acid) and activated charcoal (4.00 g/L), under dark conditions. This treatment overcame browning

Author

mpd zulkifli

Language

EN