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Gel Electrophoresis: DNA & Protein Analysis by Charm Angeles is a document available to read on EtoBox.
Gel electrophoresis separates DNA and proteins based on size. DNA is separated in agarose or acrylamide gels based on molecular weight, with smaller fragments migrating farther. Proteins are separated in polyacrylamide gels under denaturing conditions using SDS, with smaller proteins migrating farther. Ethidium bromide stains DNA, intercalating between bases and fluorescing under UV light, allowing visualization of fragments down to 0.1 μg.
- Author
- Charm Angeles
- Language
- EN