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Can I read Predicting the Partition Coefficients of a Recombinant Cutinase in Polyethylene Glycol/phosphate Aqueous Two-phase Systems on EtoBox?
Predicting the Partition Coefficients of a Recombinant Cutinase in Polyethylene Glycol/phosphate Aqueous Two-phase Systems by M. J. Sebastião; P. Martel; A. Baptista; S. B. Petersen; J. M. S. Cabral; M. R. Aires-Barros is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Predicting the Partition Coefficients of a Recombinant Cutinase in Polyethylene Glycol/phosphate Aqueous Two-phase Systems about?
A model for the prediction of protein partition coefficients in aqueous two-phase systems has been developed. This model accounts for both charge-independent and electrostatic effects. The determination of nonelectrostatic effects was based on the model of Eiteman and Gainer for uncharged solutes while the electrostatic contribution was computed using TITRA, a program that uses a continuum electrostatic model to treat charge interactions in proteins and considers the effect of pH and ionic strength. The partition coefficients of Fusarium solani pisi recombinant cutinase have been satisfactorily predicted in polyethylene glycol (PEG) 1000 and phosphate aqueous two-phase systems at a pH range of 6.0-9.0. The model failed to predict the enzyme partitioning behavior at pH 4.5.
Who reads Predicting the Partition Coefficients of a Recombinant Cutinase in Polyethylene Glycol/phosphate Aqueous Two-phase Systems?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- M. J. Sebastião; P. Martel; A. Baptista; S. B. Petersen; J. M. S. Cabral; M. R. Aires-Barros
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 1997
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)