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δ-Integration of endo/exo-glucanase and β-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol by Kwang Myung Cho; Young Je Yoo; Hyen Sam Kang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is δ-Integration of endo/exo-glucanase and β-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol about?

For the direct conversion of cellulose to ethanol, recombinant yeast having a full set of genes for the cellulolytic enzymes was developed. Using the ␦-sequences of the Ty1 retrotransposon as target sites for homologous recombination, heterologous genes of endo/exo-glucanase and ␤-glucosidase were integrated into the chromosomes of Saccharomyces cerevisiae. The number of both integrated genes was found to be approximately 44. This newly constructed yeast, S. cerevisiae L2612␦GC, successfully expressed and secreted the cellulolytic enzymes. Expression levels of cellulolytic enzymes, cell growth, and ethanol production in cellulose-containing media were significantly increased in comparison with plasmid-based expression. Maintenance of the integrated genes was also perfect up to 50 generations even in nonselectable media.

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Author

Kwang Myung Cho; Young Je Yoo; Hyen Sam Kang

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0141-0229)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)