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Survival Responses of Human Embryonic Stem Cells to DNA Damage (Article) by Tera M. Filion; Meng Qiao; Prachi N. Ghule; Matthew Mandeville; Andre J. van Wijnen; Janet L. Stein; Jane B. Lian; Dario C. Altieri; Gary S. Stein is a nonfiction available to read on EtoBox.

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## Abstract Pluripotent human embryonic stem (hES) cells require mechanisms to maintain genomic integrity in response to DNA damage that could compromise competency for lineage‐commitment, development, and tissue‐renewal. The mechanisms that protect the genome in rapidly proliferating hES cells are minimally understood. Human ES cells have an abbreviated cell cycle with a very brief G1 period suggesting that mechanisms mediating responsiveness to DNA damage may deviate from those in somatic cells. Here, we investigated how hES cells react to DNA damage induced by ionizing radiation (IR) and whether genomic insult evokes DNA repair pathways and/or cell death. We find that hES cells respond to DNA damage by rapidly inducing Caspase‐3 and ‐8, phospho‐H2AX foci, phosphorylation of p53 on Ser15 and p21 mRNA levels, as well as concomitant cell cycle arrest in G2 based on Ki67 staining and FACS analysis. Unlike normal somatic cells, hES cells and cancer cells robustly express the anti‐apoptotic protein Survivin, consistent with the immortal growth phenotype. SiRNA depletion of Survivin diminishes hES survival post‐irradiation. Thus, our findings provide insight into pathways and processes

Who reads Survival Responses of Human Embryonic Stem Cells to DNA Damage (Article)?

Author

Tera M. Filion; Meng Qiao; Prachi N. Ghule; Matthew Mandeville; Andre J. van Wijnen; Janet L. Stein; Jane B. Lian; Dario C. Altieri; Gary S. Stein

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2009

Language

EN

Category

nonfiction

Subjects

Science, Chemistry, Biology