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HLA‐C expression level in both unstimulated and stimulated human umbilical vein endothelial cells is defined by allotype by B. Sean Carey; Kay V. Poulton; Anthony Poles is a Immunology and Microbiology article available to read on EtoBox.

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Human leukocyte antigens (HLA) are present on the surface of all nucleated cells, with the level of expression dependent on the particular HLA locus, the cell type and cellular activation state. Human umbilical vein endothelial cells (HUVECs) are easily isolated from umbilical cords and may aid our understanding of HLA expression on the vascular endothelium in the setting of transplantation. Endothelial cells on the donor-recipient interface form the barrier between transplanted organs and the host immune system. Increased knowledge of the variation in levels of individual HLA specificities may inform the assessment of transplant risk. HUVECs from 48 full term babies born consecutively following planned caesarean section were isolated, HLA typed and grown on gelatin coated culture wells. Once confluent, cells were stimulated with optimal concentrations of the cytokines TNF-α and IFN-γ for 24 hours and HLA-C expression on both unstimulated and stimulated cells was quantified by flow cytometry using the fluorescent labelled monoclonal antibody DT-9 PE. Unstimulated HLA-C expression varied by over 60% between allotypes (ANOVA, P = .004). Following stimulation, HLA-C levels increased o

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Author

B. Sean Carey; Kay V. Poulton; Anthony Poles

Publisher

Wiley (Blackwell Publishing); Wiley-Blackwell; Wiley (ISSN 2059-2302)

Published

2020

Language

EN

Field

Immunology and Microbiology (Life Sciences)