Can I read Purification of guinea pig YKl40 and modulation of its secretion by cultured articular chondrocytes on EtoBox?

Purification of guinea pig YKl40 and modulation of its secretion by cultured articular chondrocytes by Frédéric De Ceuninck; Philippe Pastoureau; Françoise Bouet; Jacqueline Bonnet; Paul Michel Vanhoutte is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Purification of guinea pig YKl40 and modulation of its secretion by cultured articular chondrocytes about?

The aim of this study was to purify, characterize, and study the regulation at the chondrocyte level of the guinea pig (gp) homologue of human (R) YKL40, a putative marker of arthritic disorders. Studying YKL40 in guinea pigs is of particular interest, as age-related osteoarthritis develops in this species spontaneously. Both N-terminal sequencing and total amino acid composition of gpYKL40 purified from the secretion medium of cultured articular chondrocytes indicate a high degree of identity with hYKL40. gpYKL40 was found to contain complex N-linked carbohydrate, as demonstrated by N-glycosidase F and endoglycosidase F digestion. Isoelectric focusing demonstrated the presence of a major band at pI 6.7. The secretion of gpYKL40 by confluent articular chondrocytes in the extracellular medium was studied by immunoblotting. gpYKL40 was released by chondrocytes continuously over a 7 day period and did not appear to be degraded by proteinases, as its signal intensity in cell-free medium at 37°C did not decrease with time. Thus, gpYKL40 displays high stability and accumulates in extracellular medium without reaching a steady-state level. Among the main factors known to regulate cartilag

Who reads Purification of guinea pig YKl40 and modulation of its secretion by cultured articular chondrocytes?

Author

Frédéric De Ceuninck; Philippe Pastoureau; Françoise Bouet; Jacqueline Bonnet; Paul Michel Vanhoutte

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)