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Measurement of Amino Acid Isotope Enrichment by Liquid Chromatography Mass Spectroscopy After Derivatization with 9-Fluorenylmethylchloroformate by Hans M.H. van Eijk; Dennis P.L. Suylen; Cornelis H.C. Dejong; Yvette C. Luiking; Nicolaas E.P. Deutz is a Chemistry article available to read on EtoBox.

What is Measurement of Amino Acid Isotope Enrichment by Liquid Chromatography Mass Spectroscopy After Derivatization with 9-Fluorenylmethylchloroformate about?

An automated method is described to measure tracer-tracee-ratios (TTR) of plasma amino acids after their separation as 9fluorenylmethylchloroformate derivatives. In a 45 min cycle, 5 l plasma aliquots were derivatized, HPLC separated and subjected to electrospray ionization. By applying source collision induced dissociation, derivatives were dissociated at the peptide bond releasing the originating amino acids into the mass spectrometer. This approach enabled the determination of plasma amino acid TTRs with a standard deviation between 0.15 and 0.36%, which is sufficient to study the fate of infused tracers and their conversion products in an in vivo experiment in humans.

Who reads Measurement of Amino Acid Isotope Enrichment by Liquid Chromatography Mass Spectroscopy After Derivatization with 9-Fluorenylmethylchloroformate?

Author

Hans M.H. van Eijk; Dennis P.L. Suylen; Cornelis H.C. Dejong; Yvette C. Luiking; Nicolaas E.P. Deutz

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)

Published

2007

Language

EN

Field

Chemistry (Physical Sciences)