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Modulation of Cultured Chicken Growth Plate Chondrocytes by Transforming Growth Factor‐β~1~ and Basic Fibroblast Growth Factor by Licia N. Y. Wu; Brian R. Genge; Yoshinori Ishikawa; Dr. Roy E. Wuthier is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Modulation of Cultured Chicken Growth Plate Chondrocytes by Transforming Growth Factor‐β~1~ and Basic Fibroblast Growth Factor about?

Expression of several cellular and matrix proteins which increase significantly during the maturation of growth plate cartilage has been shown to be affected by various endocrine and autocrine factors. In the studies reported here, transforming growth factor-p (TGF-PI) and basic fibroblast growth factor (bFGF) were administered to primary cultures of avian growth plate chondrocytes at pre-or post-confluent stages to study the interplay that occurs between these factors in modulating chondrocytic phenotype. Added continuously to pre-confluent chondrocytes, TGF-P1 stimulated the cells to produce abundant extracellular matrix and multilayered cell growth; cell morphology was altered to a more spherical configuration. These effects were generally mimicked by bFGF, but cell shape was not affected. Administered together with TGF-P1, bFGF caused additive stimulation of protein synthesis, and alkaline phosphatase (AP) activity was markedly, but transiently enhanced. During this pre-confluent stage, TGF-PI also increased fibronectin secretion into the culture medium. Added to post-confluent cells, TGF-PI alone caused a dosage-dependent suppression of AP activity, but bFGF alone did not. Und

Who reads Modulation of Cultured Chicken Growth Plate Chondrocytes by Transforming Growth Factor‐β~1~ and Basic Fibroblast Growth Factor?

Author

Licia N. Y. Wu; Brian R. Genge; Yoshinori Ishikawa; Dr. Roy E. Wuthier

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1992

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)