About this Agricultural and Biological Sciences article
A Chimeric Gene (Orf256) Is Expressed as Protein Only in Cytoplasmic Male-sterile Lines of Wheat by Jiasheng Song; Charles Hedgcoth is a Agricultural and Biological Sciences article available to read on EtoBox.
Mitochondria derived from Triticum timopheevi have a chimeric gene, orf256, immediately upstream from coxI. Antibodies to a peptide corresponding to a part of the encoded amino acid sequence of orf256 detect a 7 kDa protein on western blots of mitochondrial proteins from cytoplasmic male-sterile (cms) wheat (T. aestivum nucleus, T. timopheevi mitochondria) but not in mitochondrial proteins from T. aestivum, T. timopheevi, or cms plants restored to fertility by introduction of nuclear genes for fertility res- toration. The 7 kDa protein appears to serve as a marker for cms wheat. Its occurrence as an integral protein of the inner membrane may indicate a cms effect through an influence on mitochondrial membrane function.
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- Author
- Jiasheng Song; Charles Hedgcoth
- Publisher
- Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)
- Published
- 1994
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)