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Analysis of Secondary Metabolites from Eschscholtzia Californica by High‐performance Liquid Chromatography by Maya Klvana; Jingkui Chen; François Lépine; Robert Legros; Mario Jolicoeur is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract A rapid and precise analytical HPLC method has been developed for screening the major benzophenanthridine alkaloids produced by cell cultures of __Eschscholtzia californica__, namely, sanguinarine, chelirubine, macarpine, chelerythrine and chelilutine. Separation was achieved on a C~18~ reversed‐phase column with gradient elution using acetonitrile and 50 mm phosphoric acid. Detection was performed by both fluorescence (__λ__~ex~ 330 nm, __λ__~em~ 570 nm) and photodiode array, leading to good selectivity and precision in determining peak purity. A simple and quick sample preparation protocol was elaborated involving a methanolic extraction for the measurement of intracellular concentrations of the alkaloids and a solid phase extraction for their quantification in culture medium. Owing to the non‐availability of commercially standards, a method for the purification of chelirubine, macarpine and chelilutine by semi‐preparative HPLC was developed. Coupled together, the isolation method and the analytical method were highly reliable for screening the alkaloids of interest produced by __E. californica__. Copyright © 2006 John Wiley & Sons, Ltd.

Who reads Analysis of Secondary Metabolites from Eschscholtzia Californica by High‐performance Liquid Chromatography?

Author

Maya Klvana; Jingkui Chen; François Lépine; Robert Legros; Mario Jolicoeur

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0958-0344)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)