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Dabigatran, a Direct Thrombin Inhibitor, Demonstrates Antifibrotic Effects on Lung Fibroblasts by Galina S. Bogatkevich; Anna Ludwicka-Bradley; Richard M. Silver is a Medicine article available to read on EtoBox.

What is Dabigatran, a Direct Thrombin Inhibitor, Demonstrates Antifibrotic Effects on Lung Fibroblasts about?

## Abstract ## Objective Myofibroblasts are the principal mesenchymal cells responsible for tissue remodeling, collagen deposition, and the restrictive nature of lung parenchyma associated with pulmonary fibrosis. We previously reported that thrombin activates protease‐activated receptor 1 (PAR‐1) and induces a myofibroblast phenotype in normal lung fibroblasts resembling the phenotype of scleroderma lung myofibroblasts. We undertook this study to investigate whether a selective direct thrombin inhibitor, dabigatran, interferes with signal transduction in human lung fibroblasts induced by thrombin and mediated via PAR‐1. ## Methods Lung fibroblast proliferation was analyzed using the Quick Cell Proliferation Assay. Expression and organization of α‐smooth muscle actin (α‐SMA) was studied by immunofluorescence staining and immunoblotting. Contractile activity of lung fibroblasts was measured by a collagen gel contraction assay. Connective tissue growth factor (CTGF) and type I collagen expression was analyzed on Western blots. ## Results Dabigatran, at concentrations of 50–1,000 ng/ml, inhibited thrombin‐induced cell proliferation, α‐SMA expression and organization, and the productio

Who reads Dabigatran, a Direct Thrombin Inhibitor, Demonstrates Antifibrotic Effects on Lung Fibroblasts?

Author

Galina S. Bogatkevich; Anna Ludwicka-Bradley; Richard M. Silver

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0004-3591)

Published

2009

Language

EN

Field

Medicine (Health Sciences)