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Headspace solid phase microextraction for the gas chromatographic analysis of methyl-parathion in post-mortem human samples: Application in a suicide case by intravenous injection by H Tsoukali; N Raikos; G Theodoridis; D Psaroulis is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A simple and rapid procedure for the determination of methyl-parathion (m-p) in post-mortem biological samples was developed using headspace solid phase microextraction (SPME) and gas chromatography (GC) with nitrogen-phosphorous detection (NPD). Methyl-parathion was extracted on 85 mm polyacrylate SPME fiber. Salt addition, extraction temperature, and extraction time were optimized to enhance the sensitivity of the method. The linearity (y 1⁄4 0:0473x À 0:0113, R 2 1⁄4 0:9992) and the dynamic range (0.1-40 mg/ml) were found very satisfactory. The recoveries of methyl-parathion were found to be 46% in spiked human whole blood, 53% in spiked homogenized liver tissue, and 54% in spiked homogenized kidney tissue compared with samples prepared in water. The coefficients of variations for 2, 4, and 20 mg/ml of methyl-parathion in blood ranged from 0.9 to 5.1%, whereas the detection limit of the method was satisfactory (1 ng/ml in aqueous samples, 50 ng/ml in whole blood). The developed procedure was applied to post-mortem biological samples from a 21-year-old woman fatally poisoned (suicide) by intravenous injection of methyl-parathion. The intact insecticide was found in the post-morte

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Author

H Tsoukali; N Raikos; G Theodoridis; D Psaroulis

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0379-0738)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)