Can I read DNA binding sites for putative methylation boundaries in the unmethylated region of the BRCA1 promoter on EtoBox?
DNA binding sites for putative methylation boundaries in the unmethylated region of the BRCA1 promoter by Darci T. Butcher; Debora N. Mancini‐DiNardo; Trevor K. Archer; David I. Rodenhiser is a Medicine article available to read on EtoBox.
What is DNA binding sites for putative methylation boundaries in the unmethylated region of the BRCA1 promoter about?
## Abstract Changes in DNA methylation patterns are frequently observed in human cancers and are associated with a decrease in tumor suppressor gene expression. Hypermethylation of the __BRCA1__ promoter has been reported in a portion of sporadic breast tumours that correspond to a reduction in __BRCA1__ transcription and expression. Questions remain concerning the maintenance of methylation free zones in promoter regions of tumor suppressor genes in normal tissues. Sodium bisulfite based analysis of the __BRCA1__ promoter defines a methylation free zone in normal breast tissue that starts 650 bp upstream of the transcription start site and extends for 1.4 kb through most of the __BRCA1__ CpG island. We provide data implicating 2 proteins, Sp1 and CTCF, in the maintenance of this methylation‐free zone. Both of these proteins have been shown to function as methylation boundaries in other genes. Four Sp1 sites have been identified in the __BRCA1__ promoter by gel shift assays. __In vivo__ binding of Sp1 has been confirmed at 2 of these sites in the __BRCA1__ promoter and at 2 CTCF sites that flank the unmethylated region. Our data suggest that these DNA binding sites for Sp1 and CTCF
Who reads DNA binding sites for putative methylation boundaries in the unmethylated region of the BRCA1 promoter?
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- Author
- Darci T. Butcher; Debora N. Mancini‐DiNardo; Trevor K. Archer; David I. Rodenhiser
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2004
- Language
- EN
- Field
- Medicine (Health Sciences)