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A silanized mica substrate suitable for high-resolution fiber FISH analysis by scanning near-field optical/atomic force microscopy by Shigeru Sugiyama; Megumi Fukuta; Tamaki Hirose; Toshio Ohtani; Tomoyuki Yoshino is a Engineering article available to read on EtoBox.

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## Abstract We applied a novel silanized mica substrate with an extremely flat surface constructed according to Sasou __et al__. (__Langmuir__ **19**, 9845–9849 (2003)) to high‐resolution detection of a specific gene on a DNA fiber by scanning near‐field optical/atomic force microscopy (SNOM/AFM). The interaction between the substrate and fluorescence‐dye conjugated peptide nucleic acid (PNA) probes, which causes fluorescence noise signal, was minimal. By using the substrate, we successfully obtained a fluorescence __in situ__ hybridization signal from the __ea47__ gene on a λphage DNA labeled with an Alexa 532‐conjugated 15‐base PNA probe. As the results, no fluorescence noises were observed, indicating that the surface adsorbed almost none of the PNA probe. The combination of the substrate and SNOM/AFM is an effective tool for visualizing DNA sequences at nanometer‐scale resolution. SCANNING 32: 383–389, 2010. © 2010 Wiley Periodicals, Inc.

Who reads A silanized mica substrate suitable for high-resolution fiber FISH analysis by scanning near-field optical/atomic force microscopy?

Author

Shigeru Sugiyama; Megumi Fukuta; Tamaki Hirose; Toshio Ohtani; Tomoyuki Yoshino

Publisher

Hindawi Limited; Wiley (ISSN 0161-0457)

Published

2010

Language

EN

Field

Engineering (Physical Sciences)