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A single-nucleotide mutation within the TBX3 enhancer increased body size in Chinese horses 2 Results and discussion 2 Genome sequencing and phylogenetic analysis 2 Selection signatures among horses and ponies 2 Genome-wide association study 4 Identifying TBX3 causative variant 4 Functional validation of the TBX3 causative variant 5 Supplemental information 7 Acknowledgments 7 Author contributions 7 Declaration of interests 7 References 7 STAR★Methods 10 Key resources table 10 Resource availability 12 Lead contact 12 Materials availability 12 Data and code availability 12 Experimental model and subject details 12 Candidate SNP genotyping in a larger population 12 Dual-luciferase expression assays 12 Knock-out mice preparation 12 RT-qPCR experiments 13 Bone length measurements 13 Method details 13 Pony and horse samples collection and WGS sequencing 13 Quantification and statistical analysis 14 Population structure 14 Runs of homozygosity and linkage disequilibrium 14 Selection scans 14 Genome wide association study 14 Ancient allelic trajectories 15 Transcriptome sequencing and analysis 15

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Language

UND

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nonfiction