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Hepatic Overexpression of Hemopexin Inhibits Inflammation and Vascular Stasis in Murine Models of Sickle Cell Disease by Gregory M Vercellotti; Ping Zhang; Julia Nguyen; Fuad Abdulla; Chunsheng Chen; Phong Nguyen; Carlos Nowotny; Clifford J Steer; Ann Smith; John D Belcher is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Hepatic Overexpression of Hemopexin Inhibits Inflammation and Vascular Stasis in Murine Models of Sickle Cell Disease about?

Sickle cell disease (SCD) patients have low serum hemopexin (Hpx) levels due to chronic hemolysis. We hypothesize that in SCD mice, hepatic overexpression of hemopexin will scavenge the proximal mediator of vascular activation, heme, and will inhibit inflammation and microvascular stasis. To examine the protective role of Hpx in SCD, we transplanted bone marrow from NY1DD SCD mice into Hpx or Hpx C57BL/6 mice. Dorsal skin fold chambers were implanted in week 13 post-transplant and microvascular stasis (% non-flowing venules) evaluated in response to heme infusion. Hpx sickle mice had significantly greater microvascular stasis in response to heme infusion than Hpx sickle mice (p<0.05), demonstrating the protective effect of Hpx in SCD. We utilized Sleeping Beauty (SB) transposon-mediated gene transfer to overexpress wild-type rat Hpx (wt-Hpx) in NY1DD and Townes-SS SCD mice. Control SCD mice were treated with lactated Ringer's solution (LRS) or a luciferase (Luc) plasmid. Plasma and hepatic Hpx were significantly increased compared to LRS and Luc controls. Microvascular stasis in response to heme infusion in NY1DD and Townes-SS mice overexpressing wt-Hpx had significantly less stasi

Who reads Hepatic Overexpression of Hemopexin Inhibits Inflammation and Vascular Stasis in Murine Models of Sickle Cell Disease?

Author

Gregory M Vercellotti; Ping Zhang; Julia Nguyen; Fuad Abdulla; Chunsheng Chen; Phong Nguyen; Carlos Nowotny; Clifford J Steer; Ann Smith; John D Belcher

Publisher

The Feinstein Institute for Medical Research; Springer (Biomed Central Ltd.); North Shore - Long Jewish Research Institute; Baltimore Md.: Johns Hopkins University Press; Manhasset NY: North Shore-Long Island Jewish Research Institute; Manhasset NY: Feinstein Institute for Medical Research; [London]: BioMed Central; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Test accounts (ISSN 1076-1551)

Published

2016

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)