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DNA Origami–Platelet Adducts: Nanoconstruct Binding without Platelet Activation by Yana Roka-Moiia; Vismaya Walawalkar; Ying Liu; Joseph E. Italiano; Marvin J. Slepian; Rebecca E. Taylor is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is DNA Origami–Platelet Adducts: Nanoconstruct Binding without Platelet Activation about?

Objective. Platelets are small, mechanosensitive blood cells responsible for maintaining vascular integrity and activatable on demand to limit bleeding and facilitate thrombosis. While circulating in the blood, platelets are exposed to a range of mechanical and chemical stimuli, with the platelet membrane being the primary interface and transducer of outside-in signaling. Sensing and modulating these interface signals would be useful to study mechanochemical interactions; yet, to date, no methods have been defined to attach adducts for sensor fabrication to platelets without triggering platelet activation. We hypothesized that DNA origami, and methods for its attachment, could be optimized to enable nonactivating instrumentation of the platelet membrane. Approach and Results. We designed and fabricated multivalent DNA origami nanotile constructs to investigate nanotile hybridization to membrane-embedded single-stranded DNA-tetraethylene glycol cholesteryl linkers. Two hybridization protocols were developed and validated (Methods I and II) for rendering high-density binding of DNA origami nanotiles to human platelets. Using quantitative flow cytometry, we showed that DNA origami bin

Who reads DNA Origami–Platelet Adducts: Nanoconstruct Binding without Platelet Activation?

Author

Yana Roka-Moiia; Vismaya Walawalkar; Ying Liu; Joseph E. Italiano; Marvin J. Slepian; Rebecca E. Taylor

Publisher

American Chemical Society (ACS)

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)