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Determination of Ergosterol on Mouldy Building Materials Using Isotope Dilution and Gas Chromatography–tandem Mass Spectrometry by Kristian Fog Nielsen; Jørgen Øgaard Madsen is a Chemistry article available to read on EtoBox.

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Ergosterol content of building materials was quantified using gas chromatography-tandem mass spectrometry (GC-MS-MS) in an ion trap with external ionisation. Hydrolysing the samples by classic extraction at 858C for 90 min in vials was 2 faster, more precise and safer than microwave assisted extraction. [4-H ]ergosterol was synthesised and used as internal 2 standard, giving method standard deviation of 5-10% from 10 to 30 ng to 10-15 mg ergosterol in the sample. The use of GC-MS-MS meant that no solid-phase extraction clean-up was needed, so one person could easily prepare 40-80 samples per day.

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Author

Kristian Fog Nielsen; Jørgen Øgaard Madsen

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2000

Language

EN

Field

Chemistry (Physical Sciences)