Can I read Integration of single-nuclei RNA-sequencing, spatial transcriptomics and histochemistry defines the complex microenvironment of NF1-associated plexiform neurofibromas on EtoBox?

Integration of single-nuclei RNA-sequencing, spatial transcriptomics and histochemistry defines the complex microenvironment of NF1-associated plexiform neurofibromas by Vladimir Amani; Kent A. Riemondy; Rui Fu; Andrea M. Griesinger; Enrique Grimaldo; Graziella Ribeiro De Sousa; Ahmed Gilani; Molly Hemenway; Nicholas K. Foreman; Andrew M. Donson; Nicholas Willard is a Medicine article available to read on EtoBox.

What is Integration of single-nuclei RNA-sequencing, spatial transcriptomics and histochemistry defines the complex microenvironment of NF1-associated plexiform neurofibromas about?

Plexiform neurofibroma (PN) is a leading cause of morbidity in children with the genetic condition Neurofibromatosis Type 1 (NF1), often disfiguring or threatening vital structures. During formation of PN, a complex tumor microenvironment (TME) develops, with recruitment of neoplastic and non-neoplastic cell types being critical for growth and progression. Due to the cohesive cellularity of PN, single-cell RNA-sequencing is difficult and may result in a loss of detection of critical cellular subpopulations. To bypass this barrier, we performed single-nuclei RNA-sequencing (snRNA-seq) on 8 frozen PN samples, and integrated this with spatial transcriptomics (ST) in 4 PN samples and immunohistochemistry to provide morphological context to transcriptomic data. SnRNA-seq analysis definitively charted the heterogeneous cellular subpopulations in the PN TME, with the predominant fraction being fibroblast subtypes. PN showed a remarkable amount of inter-sample homogeneity regarding cellular subpopulation proportions despite being resected from a variety of anatomical locations. ST analysis identified distinct cellular subpopulations which were annotated using snRNA-seq data and correlated

Who reads Integration of single-nuclei RNA-sequencing, spatial transcriptomics and histochemistry defines the complex microenvironment of NF1-associated plexiform neurofibromas?

Author

Vladimir Amani; Kent A. Riemondy; Rui Fu; Andrea M. Griesinger; Enrique Grimaldo; Graziella Ribeiro De Sousa; Ahmed Gilani; Molly Hemenway; Nicholas K. Foreman; Andrew M. Donson; Nicholas Willard

Publisher

Springer Science and Business Media LLC

Published

2023

Field

Medicine (Health Sciences)