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Organization, promoter analysis and transcriptional regulation of the Staphylococcus xylosus xylose utilization operon by Christine Sizemore; Eberhard Buchner; Thomas Rygus; Claudia Witke; Friedrich Götz; Wolfgang Hillen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The Staphylococcus xylosus xyl genes were cloned in Staphylococcus carnosus by complementation to xylose utilization. Xylose isomerase assays under inducing (xylose present) and non-inducing (xylose absent) conditions indicated the presence of a regulated xylA gene on the recombinant plasmid. The nucleotide sequence (4520 bases) revealed three open reading frames with the same polarity. They were identified by sequence homologies as xylR, encoding the Xyl repressor, xylA, encoding xylose isomerase and xylB, encoding xylulokinase. Primer extension analyses indicated constitutive transcription of xylR and xylose-inducible transcription of xylA. Promoter consensus sequences were found upstream of both transcriptional start sites. A transcriptional terminator between xylR and xylA separates the different transcriptional units. Potential regulatory elements were identified by sequence analysis and suggest a repressor-operator mechanism for the regulation of xylAB expression.

Who reads Organization, promoter analysis and transcriptional regulation of the Staphylococcus xylosus xylose utilization operon?

Author

Christine Sizemore; Eberhard Buchner; Thomas Rygus; Claudia Witke; Friedrich Götz; Wolfgang Hillen

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)