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Biocompatibility of NGF-grafted GTG membranes for peripheral nerve repair using cultured Schwann cells by Pei-Ru Chen; Ming-Hong Chen; Jui-Sheng Sun; Mei-Hsiu Chen; Chien-Chen Tsai; Feng-Huei Lin is a Engineering article available to read on EtoBox.
What is Biocompatibility of NGF-grafted GTG membranes for peripheral nerve repair using cultured Schwann cells about?
We previously developed a biodegradable composite with potentially good biocompatibility composed by tricalcium phosphate and gluataraldehyde cross-linking gelatin (GTG) with good mechanical property feasible for surgical manipulation. The purpose of this study was to evaluate the feasibility of immobilizing nerve growth factor (NGF) onto the composite (GTG) with carbodiimide (GEN composite). Cultured Schwann cells were seeded onto the GTG and GEN composites. For comparison, GTG membrane soaked in NGF solution without carbodiimide (GN composite) as cross-linking agent was also used to culture Schwann cells. Cell morphology was observed by a scanning electron microscope. Cell survival, cytotoxicity and cellular metabolism on the NGFgrafted GTG membrane were assessed quantitatively in terms of cell protein content, leakage of cytosolic lactate dehydrogenase (LDH) activity and by the well-established MTT assay, respectively. The result of LDH study did not show significant difference among GTG, NGF-modified GTG and control group. This indicated that GTG composite, whether cross-linking with NGF or not, has little cytotoxic effect. Comparing the protein content and MTT assay among GEN,
Who reads Biocompatibility of NGF-grafted GTG membranes for peripheral nerve repair using cultured Schwann cells?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Pei-Ru Chen; Ming-Hong Chen; Jui-Sheng Sun; Mei-Hsiu Chen; Chien-Chen Tsai; Feng-Huei Lin
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)
- Published
- 2004
- Language
- EN
- Field
- Engineering (Physical Sciences)