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Synthesis of Functionalized Rab GTPases by a Combination of Solution- or Solid-Phase Lipopeptide Synthesis with Expressed Protein Ligation by Luc Brunsveld; Anja Watzke; Thomas Durek; Kirill Alexandrov; Roger S. Goody; Herbert Waldmann is a Chemistry article available to read on EtoBox.
What is Synthesis of Functionalized Rab GTPases by a Combination of Solution- or Solid-Phase Lipopeptide Synthesis with Expressed Protein Ligation about?
## Abstract Prenylated proteins with non‐native functionalities are generally very difficult to obtain by recombinant or enzymatic means. The semisynthesis of preparative amounts of prenylated Rab guanosine triphosphatases (GTPases) from recombinant proteins and synthetic prenylated peptides depends largely on the availability of functionalised prenylated peptides corresponding to the proteins' native structure or modifications thereof. Here, we describe and compare solution‐phase and solid‐phase strategies for the generation of peptides corresponding to the prenylated C terminus of Rab7 GTPase. The solid‐phase with utilisation of a hydrazide linker emerges as the more favourable approach. It allows a fast and practical synthesis of pure peptides and gives a high degree of flexibility in their modification. To facilitate the analysis of semisynthetic proteins, the synthesised peptides were equipped with a fluorescent group. Using the described approach, we introduced fluorophores at several different positions of the Rab7 C terminus. The position of the incorporated fluorescent groups in the peptides did not influence the protein‐ligation reaction, as the generated peptides could b
Who reads Synthesis of Functionalized Rab GTPases by a Combination of Solution- or Solid-Phase Lipopeptide Synthesis with Expressed Protein Ligation?
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- Author
- Luc Brunsveld; Anja Watzke; Thomas Durek; Kirill Alexandrov; Roger S. Goody; Herbert Waldmann
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0947-6539)
- Published
- 2005
- Language
- EN
- Field
- Chemistry (Physical Sciences)