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Can I read Molecular Analysis of Barley Mutants Deficient in Chloroplast Glutamine Synthetase on EtoBox?
Molecular Analysis of Barley Mutants Deficient in Chloroplast Glutamine Synthetase by Jacqueline Freeman; Antonio J. Marquez; Roger M. Wallsgrove; Ritva Saarelainen; Brian G. Forde is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Molecular Analysis of Barley Mutants Deficient in Chloroplast Glutamine Synthetase about?
A barley leaf cDNA library has been screened with two oligonucleotide probes designed to hybridize to conserved sequences in glutamine synthetase (GS) genes from higher plants. Two GS cDNA clones were identified as hybridizing strongly to one or both probes. The larger clone (pcHvGS6) contained a 1.6 kb insert which was shown by primer extension analysis to be an almost full-length cDNA. Both clones were more closely related to cDNAs for the chloroplast form of GS (GS2) from pea and Phaseolus vulgaris than to cDNAs for the cytosolic form (GS1). A sequence identical to an N-terminal sequence determined from a purified preparation of the mature GS2 polypeptide (NH2-XLGPETTGVIQRMQQ) was found in the pcHvGS6-encoded polypeptide at residues 46-61, indicating a pre-sequence of at least 45 amino acids. The pre-sequence has only limited sequence homology to the pre-sequences of pea and P. vulgaris G S 2 subunits, but is similarly rich in basic residues and possesses some of the structural features common to the targeting sequences of other chloroplast proteins. The molecular lesions responsible for the GS2-deficient phenotypes of eight photorespiratory mutants of barley were investigated u
Who reads Molecular Analysis of Barley Mutants Deficient in Chloroplast Glutamine Synthetase?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Jacqueline Freeman; Antonio J. Marquez; Roger M. Wallsgrove; Ritva Saarelainen; Brian G. Forde
- Publisher
- Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)
- Published
- 1990
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)