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Can I read Structural Characterization and Anti‐angiogenic Properties of Prostate‐specific Antigen Isoforms in Seminal Fluid on EtoBox?
Structural Characterization and Anti‐angiogenic Properties of Prostate‐specific Antigen Isoforms in Seminal Fluid by Johanna M. Mattsson; Leena Valmu; Pirjo Laakkonen; Ulf‐Håkan Stenman; Hannu Koistinen is a Medicine article available to read on EtoBox.
What is Structural Characterization and Anti‐angiogenic Properties of Prostate‐specific Antigen Isoforms in Seminal Fluid about?
## Abstract ## BACKGROUND The prostate produces high levels of prostate‐specific antigen (PSA), which has been shown to exert anti‐angiogenic properties and thus might slow down prostate tumor growth. It has been suggested that the protease activity of PSA is not needed for its anti‐angiogenic function. We have previously shown that seminal fluid contains both active and inactive, internally cleaved forms of PSA. The precise structural differences between these isoforms and their function are not known. ## METHODS To elucidate the structures, we purified PSA from seminal fluid and separated it by anion‐exchange chromatography into six different isoforms, which were characterized by mass spectrometry. The anti‐angiogenic activity of these PSA‐isoforms was analyzed by human umbilical vein endothelial cell (HUVEC) tube formation assay. ## RESULTS The enzymatically active PSA‐isoforms had an intact peptide moiety but could be separated into three isoforms based on differences in glycosylation. The major isoform contained PSA with a biantennary carbohydrate with terminal sialic acids on both antennae. The other active isoforms showed significant carbohydrate heterogeneity, containing on
Who reads Structural Characterization and Anti‐angiogenic Properties of Prostate‐specific Antigen Isoforms in Seminal Fluid?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Johanna M. Mattsson; Leena Valmu; Pirjo Laakkonen; Ulf‐Håkan Stenman; Hannu Koistinen
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-4137)
- Published
- 2008
- Language
- EN
- Field
- Medicine (Health Sciences)