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Can I read Cognate base‐pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase on EtoBox?
Cognate base‐pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase by Kimoto, Michiko; Soh, Si Hui Gabriella; Tan, Hui Pen; Okamoto, Itaru; Hirao, Ichiro is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Cognate base‐pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase about?
We present cognate base pair selectivity in template-dependent ligation by T4 DNA ligase using a hydrophobic unnatural base pair (UBP), Ds-Pa. T4 DNA ligase efficiently recognizes the Ds-Pa pairing at the conjugation position, and Ds excludes the noncognate pairings with the natural bases. Our results indicate that the hydrophobic base pairing is allowed in enzymatic ligation with higher cognate base-pair selectivity, relative to the hydrogen-bond interactions between pairing bases. The efficient ligation using Ds-Pa can be employed in recombinant DNA technology using genetic alphabet expansion, toward the creation of semi-synthetic organisms containing UBPs.
Who reads Cognate base‐pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kimoto, Michiko; Soh, Si Hui Gabriella; Tan, Hui Pen; Okamoto, Itaru; Hirao, Ichiro
- Publisher
- Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0006-3525)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)