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Protective effect of the DNA vaccine encoding the major house dust mite allergens on allergic inflammation in the murine model of house dust mite allergy by Nacksung Kim; Soon Seog Kwon; Jaechun Lee; Sohyung Kim; Tai June Yoo is a Medicine article available to read on EtoBox.

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## Background Vaccination with naked DNA encoding antigen induces cellular and humoral immunity characterized by the activation of specific Th1 cells. ## Objective To evaluate the effects of vaccination with mixed naked DNA plasmids encoding __Der p__ 1, __Der p__ 2, __Der p__ 3, __Der f__ 1, __Der f__ 2, and __Der f__ 3, the major house dust mite allergens on the allergic inflammation to the whole house dust mites (HDM) crude extract. ## Methods Three hundred micrograms of these gene mixtures were injected into muscle of BALB/c mice. Control mice were injected with the pcDNA 3.1 blank vector. After 3 weeks, the mice were actively sensitized and inhaled with the whole house dust mite extract intranasally. ## Results The vaccinated mice showed a significantly decreased synthesis of total and HDM-specific IgE compared with controls. Analysis of the cytokine profile of lymphocytes after challenge with HDM crude extract revealed that mRNA expression of interferon-γ was higher in the vaccinated mice than in the controls. Reduced infiltration of inflammatory cells and the prominent infiltration of CD8+ T cells were observed in histology of lung tissue from the vaccinated mice. ## Conclus

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Author

Nacksung Kim; Soon Seog Kwon; Jaechun Lee; Sohyung Kim; Tai June Yoo

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, c2003-; Springer Science and Business Media LLC (ISSN 1476-7961)

Published

2006

Language

EN

Field

Medicine (Health Sciences)