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A glucose biosensor based on modified-enzyme incorporated within electropolymerised poly(3,4-ethylenedioxythiophene) (PEDT) films by Benoı̂t Piro; Lan Anh Dang; Minh Chau Pham; Silvia Fabiano; Canh Tran-Minh is a Engineering article available to read on EtoBox.

What is A glucose biosensor based on modified-enzyme incorporated within electropolymerised poly(3,4-ethylenedioxythiophene) (PEDT) films about?

We have constructed and characterised a glucose sensor using glucose oxidase (GOD) covalently attached to carboxylic acid polyethyleneglycol (PEG), called (PEG-GOD). This modified enzyme was entrapped afterwards within poly(3,4-ethylenedioxythiophene) (PEDT) films electrogenerated on glassy carbon (GC) electrodes. The composite (PEG-GOD/PEDT) film is more porous than the film without enzyme (PEDT + PEG). Data from electrochemical quartz microbalance (ECQM) and pH-stat experiments indicate a good relative activity of the modified enzyme, ca. 12 -15%. Amperometric measurements, using ferrocenemethanol as the redox mediator, confirms that the modified enzyme is catalytically active. The effect of film thickness was also investigated. The sensitivities were quite similar for modified-GOD electrodes (ca. 3 mA cm -2 M -1 ) and unmodified-GOD electrodes (ca. 2.7 mA cm -2 M -1 ) but a better stability was obtained with modified PEG-GOD electrodes.

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Author

Benoı̂t Piro; Lan Anh Dang; Minh Chau Pham; Silvia Fabiano; Canh Tran-Minh

Publisher

Elsevier ; Elsevier BV (ISSN 1572-6657)

Published

2001

Language

EN

Field

Engineering (Physical Sciences)