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Underlying mechanisms of apoptosis in HepG2 cells induced by polyphyllin I through Fas death and mitochondrial pathways by Yawen Zeng; Zhiqin Zhang; Wenping Wang; Longtai You; Xiaoxv Dong; Xingbin Yin; Changhai Qu; Jian Ni is a Environmental Science article available to read on EtoBox.
What is Underlying mechanisms of apoptosis in HepG2 cells induced by polyphyllin I through Fas death and mitochondrial pathways about?
Polyphyllin I, a steroidal saponin in which possess broad application prospects in cancer prevention and treatment. The purpose of this study was to determine the potential cytotoxicity and mechanism of Polyphyllin I in HepG2 cells. In this study, we used MTT to evaluate cell survival. Cell apoptosis rate, cell cycle distribution, mitochondrial membrane potential and ros levels were measured by flow cytometry, and the expression of apoptosis-related proteins was determined by Western blot analysis. Polyphyllin I significantly reduced cell viability and induced HepG2 cell apoptosis in a dose and time-dependent manner. Compared with the control group, it could induce reactive oxygen species (ROS) generation and depolarization of matrix metalloproteinases in liver cells. Polyphyllin I dose-dependent increased the release of mitochondrial cytochrome c, and levels of Fas, p53, p21, and Bax/Bcl-2 ratios, as well as the activation of cleaved caspase-3, -8, -9, and subsequent cleavage of the poly (ADP-ribose) polymerase (PARP). The G2/M phase cell cycle arrest was induced by increasing the expression of p21 and cyclin E1, and significantly reducing the expression of cyclin A2 and CDK2. Our
Who reads Underlying mechanisms of apoptosis in HepG2 cells induced by polyphyllin I through Fas death and mitochondrial pathways?
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Yawen Zeng; Zhiqin Zhang; Wenping Wang; Longtai You; Xiaoxv Dong; Xingbin Yin; Changhai Qu; Jian Ni
- Publisher
- Informa UK Limited
- Published
- 2020
- Language
- EN
- Field
- Environmental Science (Life Sciences)