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Catalase Activity Estimation Protocol by Säthèésh Mãŕłëý is a document available to read on EtoBox.
What is Catalase Activity Estimation Protocol about?
The document outlines a procedure for estimating catalase enzyme activity, which converts hydrogen peroxide (H2O2) into water and oxygen, thus reducing plant toxicity and enhancing drought tolerance. It details the reagents required, including phosphate buffer and sodium perborate, and provides a step-by-step procedure for macerating leaf samples, centrifuging, and titrating against potassium permanganate. The final enzyme activity is calculated based on the volume of KMnO4 consumed, expressed as μg of H2O2
- Author
- Säthèésh Mãŕłëý
- Language
- EN