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What is RP-HPLC Method for Methylcobalamin Estimation about?

This research paper describes the development and validation of a reverse phase high performance liquid chromatography (RP-HPLC) method for the estimation of methylcobalamin in bulk drug and capsule dosage forms. The method utilizes a C18 column with a mobile phase of acetonitrile and sodium dihydrogen ortho phosphate (20:80) at pH 3.5. Methylcobalamin was detected at 376nm with a retention time of 7.8 minutes. The method was validated per ICH guidelines and was found to be linear, precise, accurate, robust

Author

Layli Amalia

Language

EN