About this Medicine article

Enhanced degradation of proteins of the basal lamina and stroma by matrix metalloproteinases from the salivary glands of Sjögren's syndrome patients: Correlation with reduced structural integrity of acini and ducts by Eduardo Goicovich; Claudio Molina; Paola Pérez; Sergio Aguilera; Juan Fernández; Nancy Olea; Cecilia Alliende; Cecilia Leyton; Rafael Romo; Lisette Leyton; María‐Julieta González is a Medicine article available to read on EtoBox.

## Abstract ## Objective To determine the effect of matrix metalloproteinase (MMP) activity from the labial salivary glands (LSGs) of Sjögren's syndrome (SS) patients on proteins of the extracellular matrix (ECM) that form the basal lamina and stroma, and to compare this effect with the structural integrity of acini and ducts as well as the functionality of the LSGs. ## Methods Gelatinase activity was determined by zymography. The digestion pattern of extracellular matrix (ECM) macromolecules was detected by gel electrophoresis and quantified by densitometry. The structural integrity of acini and ducts was evaluated by light and electron microscopy. Secretory function was evaluated by measuring unstimulated salivary flow and by scintigraphy. ## Results LSG extracts showed increased levels of proteolytic activity toward purified proteins of the basal lamina (laminin and type IV collagen) and stroma (types I and III collagen and fibronectin). Enhanced degradation was most evident for fibronectin, laminin, and type IV collagen. Analysis of the ultrastructure of the acinar and ductal basal lamina revealed abnormalities ranging from disorganization to disappearance of this ECM structure

Author

Eduardo Goicovich; Claudio Molina; Paola Pérez; Sergio Aguilera; Juan Fernández; Nancy Olea; Cecilia Alliende; Cecilia Leyton; Rafael Romo; Lisette Leyton; María‐Julieta González

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0004-3591)

Published

2003

Language

EN

Field

Medicine (Health Sciences)