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Cobalt(II)-substituted carboxypeptidase A has been found to reversibly bind N3-and NCO-, but not NCS, in the pH range 5-10, thus including the pH range of activity of the enzyme. The pH dependence of the anion binding constant is affected by two ionizations, which are assigned as those regulating kcat and KM. The electronic and 'H NMR spectra are consistent with a substantially pseudotetrahedral geometry of the anion derivatives.

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Author

I. Bertini; G. Lanini; C. Luchinat; R. Monnanni

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0020-1693)

Published

1985

Language

EN

Field

Chemistry (Physical Sciences)