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Physiological and biochemical improvement of the enzyme side-chain degradation of cholesterol by Fusarium solani by Lotfy A.R. Sallam; Abdel-Monem El-Refai; Hassan A. El-Minofi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The conversion of cholesterol (C) to androstenedione (AD) and androstadienedione (ADD) was influenced significantly by the composition of the cultivation medium. A medium composed of (g/l): glucose, 50; peptone, 20; corn steep solid, 3; supported relatively high AD and ADD outputs. C convertibility was further accelerated when the cultivation medium was adjusted with phosphate buffer with the pH range 5.5-7.38. Supplementation of the medium with 8-hydroxyquinoline or methanol prevented the degradation of the C nucleus with concomitant formation of good AD and ADD yields.

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Author

Lotfy A.R. Sallam; Abdel-Monem El-Refai; Hassan A. El-Minofi

Publisher

Elsevier Science; Elsevier ; Elsevier Applied Science; Elsevier BV (ISSN 1873-3298)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)