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Ca^2+^ Responses to Acetylcholine and Adenosine Triphosphate in the Otocyst of Chick Embryo by Yoshikazu Nakaoka; Masayuki Yamashita is a Neuroscience article available to read on EtoBox.

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The action of acetylcholine and adenosine triphosphate (ATP) on cytoplasmic Ca2+ concentration ([Ca2+Ji) was studied in the otocyst epithelium of embryonic day 3 chicks with Ca2+-sensitive fluorescence measurements. Increases in [Ca2+li were evoked by the bath application of acetylcholine (1 pM or higher). The rise in [Ca2+Ii was due to the release of Ca2+ from intracellular Ca2+ stores, since the Ca2+ response occurred even in a Ca2+-free medium. The Ca2+ response to acetylcholine was mediated by muscarinic receptors. Atropine of 1 pM abolished the response to 10 pM acetylcholine; muscarine and carbamylcholine (100 pM each) evoked Ca2+ rises. Increases in [Ca2+li were also evoked by the bath application of ATP (10 pM or higher). The Ca2+ rise by ATP was evoked even in a Ca2+-free medium. Adenosine (500 p M ) did not cause any Ca2+ response. Suramin and reactive blue 2 (200 pM each) completely blocked the Ca2' response to 500 pM ATP. Uridine triphosphate (500 puM) caused comparable Ca2+ responses with those to 500 pM ATP. These results suggested the involvement of P2" purinoceptors. The potentiation of CaZ+ rise was observed when acetylcholine and ATP were co-applied at submaximal

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Author

Yoshikazu Nakaoka; Masayuki Yamashita

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3034)

Published

1995

Language

EN

Field

Neuroscience (Life Sciences)