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A Low-volume Platform for Cell-respirometric Screening Based on Quenched-luminescence Oxygen Sensing by John Alderman; James Hynes; Suzanne M Floyd; Jan Krüger; Rosemary O’Connor; Dmitri B Papkovsky is a Engineering article available to read on EtoBox.

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Cell viability assays represent an important technology in modern cell biology, drug discovery and biotechnology, where currently there is a high demand for simple, sensitive and cost-effective screening methods. We have developed a new methodology and associated tools for cell-based screening assays, which are based on the measurement of the rates of oxygen uptake in cells by luminescence quenching. Sealable microchamber devices matching the footprint of a standard 96-well plate were developed and used in conjunction with long-decay phosphorescent oxygen probes. These devices permit cell non-invasive, real-time monitoring of cellular respiration and a rapid, one-step, kinetic assessment of multiple samples for cell viability, drug/effector action. These assays can be carried out on conventional fluorescence plate readers, they are suitable for different types of cells, including adherent and slow-respiring cells, require small sample volumes and cell numbers, and are amenable for high throughput screening. Monitoring of as little as 300 mammalian cells in 3 l volume has been demonstrated.

Who reads A Low-volume Platform for Cell-respirometric Screening Based on Quenched-luminescence Oxygen Sensing?

Author

John Alderman; James Hynes; Suzanne M Floyd; Jan Krüger; Rosemary O’Connor; Dmitri B Papkovsky

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2004

Language

EN

Field

Engineering (Physical Sciences)