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Cryotherapy Modifies Synthetic Activity and Differentiation of Keloidal Fibroblasts in Vitro by A. Dalkowski; S. Fimmel; C. Beutler; Ch. C. Zouboulis is a Medicine article available to read on EtoBox.

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**Abstract:** In order to obtain a persuasive explanation for the beneficial clinical effect of cryotherapy on keloids, we developed a reproducible model to apply freezing temperatures on cell cultures, and investigated their influence on proliferation, viability, synthetic activity and differentiation of dermal fibroblasts __in vitro__. Cell cultures were established from 13 untreated keloids and 10 healthy skin specimens matched for age and skin localization to the donors. No significant influence of cell freezing on the proliferation rates of both keloidal and normal fibroblasts was documented, but mechanical cell destruction with a wide variation in lethality rates (29% average lethal effect on keloidal fibroblasts and 41% on normal ones) was observed. When comparing specimens of keloidal and normal tissue derived from the same four donors, the keloidal fibroblasts were similar regarding their synthetic activity but presented enhanced tenascin‐C expression compared with the normal fibroblasts. After cryotherapy, delayed collagen III increase was detected in both cell types (__P__ = 0.03). The collagen III/collagen I ratio increased from 1.6 to 2.8 in the keloidal and only from

Who reads Cryotherapy Modifies Synthetic Activity and Differentiation of Keloidal Fibroblasts in Vitro?

Author

A. Dalkowski; S. Fimmel; C. Beutler; Ch. C. Zouboulis

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0906-6705)

Published

2003

Language

EN

Field

Medicine (Health Sciences)