Opening book details…
Can I read Disruption of six novel ORFs on the left arm of chromosome XII reveals one gene essential for vegetative growth ofSaccharomyces cerevisiae on EtoBox?
Disruption of six novel ORFs on the left arm of chromosome XII reveals one gene essential for vegetative growth ofSaccharomyces cerevisiae by Zhang, Nianshu; Ismail, Thamir; Wu, Jian; Woodwark, K. Cara; Gardner, David C. J.; Walmsley, Richard M.; Oliver, Stephen G. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Disruption of six novel ORFs on the left arm of chromosome XII reveals one gene essential for vegetative growth ofSaccharomyces cerevisiae about?
Deletion via PCR-mediated gene replacement, together with basic functional and bioinformatic analyses, have been performed on six novel open reading-frames (ORFs) on the left arm of chromosome XII of Saccharomyces cerevisiae (YLL033w, YLL032c, YLL031c, YLL030c, YLL029w and YLL028w). ORF deletion was realized using either a short-flanking homology (SFH) or a long-flanking homology (LFH) replacement cassette in the diploid strain FY1679. Sporulation and tetrad analysis showed that YLL031c is the only essential gene of the six. Microscopic examination of the non-growing spores carrying a disrupted copy of the essential gene showed that most of them were blocked after one or two cell divisions with heterogeneous bud size. The standard EUROFAN growth tests failed to reveal any obvious phenotype resulting from the deletion of each the five non-essential ORFs. Bioinformatic analysis revealed that YLL029w is probably an aminopeptidase for mitochondrial or nuclear protein processing and YLL028w may be involved in drug resistance in S. cerevisiae. Replacement cassettes, comprising the promoter and terminator regions of each of the six ORFs, were cloned into pUG7 and demonstrated to efficient
Who reads Disruption of six novel ORFs on the left arm of chromosome XII reveals one gene essential for vegetative growth ofSaccharomyces cerevisiae?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Zhang, Nianshu; Ismail, Thamir; Wu, Jian; Woodwark, K. Cara; Gardner, David C. J.; Walmsley, Richard M.; Oliver, Stephen G.
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)
- Published
- 1999
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)