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Characterization of a novel modification on IgG2 light chain: Evidence for the presence of O-linked mannosylation by Theresa Martinez; Danielle Pace; Lowell Brady; Mary Gerhart; Alain Balland is a Chemistry article available to read on EtoBox.

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This paper describes the analysis of a novel modification identified on the light chain of a recombinant IgG2 antibody. This modification, a +162 Da adduct, suggestive of a single hexose addition, was observed by mass analysis of the reduced molecule. The modification was located on residue serine 66 of the light chain by investigation with LC-MS peptide mapping, mass spectrometry and N-terminal sequencing techniques. Location of the adduct on serine pointed the investigation toward O-linked glycosylation. Identification of the hexose residue was deduced from its elimination by action of ␣-mannosidase, providing evidence for the presence of an O-mannosylated light chain. This type of modification in the glycosylation profile of antibodies, to our knowledge, has not been reported for human IgG molecules.

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Author

Theresa Martinez; Danielle Pace; Lowell Brady; Mary Gerhart; Alain Balland

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2007

Language

EN

Field

Chemistry (Physical Sciences)