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Can I read A simple procedure for the isolation of l-fucose-binding lectins from Ulex europaeus and Lotus tetragonolobus on EtoBox?
A simple procedure for the isolation of l-fucose-binding lectins from Ulex europaeus and Lotus tetragonolobus by Howard J. Allen; Edward A.Z. Johnson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A simple procedure for the isolation of l-fucose-binding lectins from Ulex europaeus and Lotus tetragonolobus about?
r.-Fucose-bmdmg lectms from UZex europaeus and Lutzrs tetragonolobus were Isolated by ai&uty chromatography on columns of L-fucose-Sepbarose 6B L-Fucose was coupled to Sepharose 6B after dlvmyl sulfone-actlvatlon of the gel to gee an afEmty adsorbent capable of binding more than 1 2 mg of U/ex Iectm/ml of gel, which could then be eluted with 0 lar or 0 05~ L-fucose Analysis of the isolated lectms by hemagglutmatlon assay, by gel filtration, and by polyacrylamlde discelectrophoresls revealed the presence of lsolectms, or aggregated species, or both The apparent mol wt of the maJor lectm fraction from Lotus was 35 GO0 when determined on Sephadex G-200 or Ultrogel AcA 34 In contrast, the apparent mol wt of the maJor lectm fraction from Ulex was 68 000 when chromatographed on Sephadex G-200 and 45 000 when chromatographed on Ultroge! AcA 34 The yields of lectms were 4 5 mg/lOO g of Ulex seeds and 394 mg/lOO g of Lotus seeds
Who reads A simple procedure for the isolation of l-fucose-binding lectins from Ulex europaeus and Lotus tetragonolobus?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Howard J. Allen; Edward A.Z. Johnson
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)
- Published
- 1977
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)