Can I read Identification of a Monoclonal Antibody Clipping Variant by Cross-validation Using Capillary Electrophoresis – Sodium Dodecyl Sulfate, Capillary Zone Electrophoresis – Mass Spectrometry and Capillary Isoelectric Focusing – Mass Spectrometry on EtoBox?

Identification of a Monoclonal Antibody Clipping Variant by Cross-validation Using Capillary Electrophoresis – Sodium Dodecyl Sulfate, Capillary Zone Electrophoresis – Mass Spectrometry and Capillary Isoelectric Focusing – Mass Spectrometry by Meng Li; Xueyu Zhao; Danfeng Shen; Gang Wu; Wenbo Wang; Chuanfei Yu; John Sausen; Hanmei Xu; Lan Wang is a Chemistry article available to read on EtoBox.

What is Identification of a Monoclonal Antibody Clipping Variant by Cross-validation Using Capillary Electrophoresis – Sodium Dodecyl Sulfate, Capillary Zone Electrophoresis – Mass Spectrometry and Capillary Isoelectric Focusing – Mass Spectrometry about?

Critical quality attributes (CQAs) of recombinant monoclonal antibody therapeutics are constantly monitored throughout the life cycle of drug development and manufacturing. In the past few decades, numerous analytical techniques have been developed for the characterization of CQAs. In this regard, non-reduced and reduced capillary electrophoresis – sodium dodecyl sulfate (CE-SDS) methods have been widely adopted by the biopharmaceutical industry for the evaluation of size-related heterogeneities in biologics. In this work we demonstrate that, with recent development of capillary electrophoresis – mass spectrometry (CE-MS) technologies, a clipping variant of bevacizumab may be identified directly by both capillary zone electrophoresis – mass spectrometry (CZE-MS) and capillary isoelectric focusing – mass spectrometry (cIEF-MS) approaches, providing a powerful addition to the traditional CE-SDS analysis workflow. In this novel workflow, linear regression between the mobility and molecular weight first results in an approximate size range of this variant. The intact masses of all species in the bevacizumab are then obtained, after deconvolution of all features identified in the CZE-MS

Who reads Identification of a Monoclonal Antibody Clipping Variant by Cross-validation Using Capillary Electrophoresis – Sodium Dodecyl Sulfate, Capillary Zone Electrophoresis – Mass Spectrometry and Capillary Isoelectric Focusing – Mass Spectrometry?

Author

Meng Li; Xueyu Zhao; Danfeng Shen; Gang Wu; Wenbo Wang; Chuanfei Yu; John Sausen; Hanmei Xu; Lan Wang

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Chemistry (Physical Sciences)