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Butyrate suppresses tumor necrosis factor α production by regulating specific messenger RNA degradation mediated through a cis ‐acting AU‐rich element by Jun Fukae; Yoshiharu Amasaki; Yumi Yamashita; Toshiyuki Bohgaki; Shinsuke Yasuda; Satoshi Jodo; Tatsuya Atsumi; Takao Koike is a Medicine article available to read on EtoBox.
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## Abstract ## Objective To study the capacity of butyrate to inhibit production of tumor necrosis factor α (TNFα) in macrophage‐like synoviocytes (MLS) from patients with rheumatoid arthritis (RA), in human peripheral monocytes, and in murine RAW264.7 macrophages. ## Methods The concentrations of TNFα in culture supernatants of these cells were measured using enzyme‐linked immunosorbent assay. The expression levels of various messenger RNAs (mRNA), such as those for TNFα, the mRNA‐binding protein TIS11B, and luciferase, were measured using real‐time quantitative polymerase chain reaction. The in vitro effects of butyrate on transcriptional regulation were evaluated by transfection with various reporter plasmids in RAW264.7 macrophages. The effects of TIS11B on TNFα expression were examined using an overexpression model of TIS11B in RAW264.7 cells. ## Results Butyrate suppressed TNFα protein and mRNA production in MLS and monocytes, but paradoxically enhanced transactivation of the TNFα promoter. Expression of the AU‐rich element (ARE)–binding protein TIS11B was up‐regulated by butyrate. Induction of TNFα mRNA by lipopolysaccharide was significantly inhibited when TIS11B was overex
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- Author
- Jun Fukae; Yoshiharu Amasaki; Yumi Yamashita; Toshiyuki Bohgaki; Shinsuke Yasuda; Satoshi Jodo; Tatsuya Atsumi; Takao Koike
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0004-3591)
- Published
- 2005
- Language
- EN
- Field
- Medicine (Health Sciences)