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Early-life antibiotic interventions can change the predisposition to disease by disturbing the gut microbiota. Differences in Microbiota Membership along the Gastrointestinal Tract of Piglets and Their Differential Alterations Following an Early-Life Antibiotic Intervention 1 Introduction 1 Materials And Methods 2 Animal Husbandry 2 Slaughtering and Sampling 3 DNA Extraction, 16S rRNA Gene Amplification, and High-Throughput Sequencing 3 Bioinformatics Analysis of Sequencing Data 3 Prediction of Microbial Metagenomes 3 Statistical Analysis 4 Accession Number 4 Results 4 Summary of High-Throughput Sequencing Data 4 Impact of Gut Location, Lumen/Mucosa, and Antibiotics on the Bacterial Composition 4 Bacterial Taxa Affected by the Early-Life Antibiotic Intervention 4 Effect of the Antibiotic Intervention on the Representative Dominant OTUs in Piglets 6 Effect of Antibiotics on Microbial Functional Capability 6 Discussion 8 Spatial Variations in the Microbiota of the Stomach versus the Intestine and Lumen versus the Mucosa 8 Differential and Compartmentalized Alterations in Gut Microbiota in Response to the Early-Life Antibiotic Intervention 10 Conclusion 12 Author Contributions 12 Ackn

Author

Weiyun Zhu

Language

EN