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Nucleoside triphosphate diphosphohydrolase‐2 is the ecto‐ATPase of type I cells in taste buds by Dianna L. Bartel; Susan L. Sullivan; Élise G. Lavoie; Jean Sévigny; Thomas E. Finger is a Neuroscience article available to read on EtoBox.

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## Abstract The presence of one or more calcium‐dependent ecto‐ATPases (enzymes that hydrolyze extracellular 5′‐triphosphates) in mammalian taste buds was first shown histochemically. Recent studies have established that dominant ecto‐ATPases consist of enzymes now called __nucleoside triphosphate diphosphohydrolases__ (NTPDases). Massively parallel signature sequencing (MPSS) from murine taste epithelium provided molecular evidence suggesting that NTPDase2 is the most likely member present in mouse taste papillae. Immunocytochemical and enzyme histochemical staining verified the presence of NTPDase2 associated with plasma membranes in a large number of cells within all mouse taste buds. To determine which of the three taste cell types expresses this enzyme, double‐label assays were performed with antisera directed against the glial glutamate/aspartate transporter (GLAST), the transduction pathway proteins phospholipase Cβ2 (PLCβ2) or the G‐protein subunit α‐gustducin, and serotonin (5HT) as markers of type I, II, and III taste cells, respectively. Analysis of the double‐labeled sections indicates that NTPDase2 immunoreactivity is found on cell processes that often envelop other ta

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Author

Dianna L. Bartel; Susan L. Sullivan; Élise G. Lavoie; Jean Sévigny; Thomas E. Finger

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0021-9967)

Published

2006

Language

EN

Field

Neuroscience (Life Sciences)