Can I read Salamander‐Eci : An optical clearing protocol for the three‐dimensional exploration of regeneration on EtoBox?
Salamander‐Eci : An optical clearing protocol for the three‐dimensional exploration of regeneration by Subiran Adrados, Cristina; Yu, Qinghao; Bolaños Castro, Lizbeth Airais; Rodriguez Cabrera, Luis Alberto; Yun, Maximina is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Salamander‐Eci : An optical clearing protocol for the three‐dimensional exploration of regeneration about?
## Background: Salamander limb regeneration is a complex biological process that entails the orchestration of multiple cellular and molecular mechanisms in a three-dimensional space. hence, a comprehensive understanding of this process requires whole-structure level explorations. recent advances in imaging and optical clearing methods have transformed the study of regenerative phenomena, allowing the three-dimensional visualization of structures and entire organisms. ## Results: Here we introduce salamander-eci, a rapid and robust optical clearing protocol optimized for the widely used axolotl model, which allows simultaneous immunohistochemistry and click-chemistry detection with minimal volume disruption. we provide examples of its application, from whole larva to adult limbs and organs, and complement it with an image analysis pipeline for volumetric cell quantification. further, we offer a detailed 3d quantitation of cell proliferation throughout axolotl limb regeneration. ## Conclusions: Salamander-eci enables the comprehensive volumetric analysis of regenerative phenomena at both local and systemic levels.
Who reads Salamander‐Eci : An optical clearing protocol for the three‐dimensional exploration of regeneration?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Subiran Adrados, Cristina; Yu, Qinghao; Bolaños Castro, Lizbeth Airais; Rodriguez Cabrera, Luis Alberto; Yun, Maximina
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1058-8388)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)