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Ultra-performance Liquid Chromatography/tandem Mass Spectrometric Determination of Testosterone and Its Metabolites in in Vitro Samples by Ganfeng Wang; Yunsheng Hsieh; Xiaoming Cui; Kuo-Chi Cheng; Walter A. Korfmacher is a Chemistry article available to read on EtoBox.

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## Abstract This paper describes the development and partial validation of a fast, sensitive and specific ultra‐performance liquid chromatography/tandem mass spectrometric (UPLC/MS/MS) method for the determination of testosterone (T) and its four metabolites, 6__β__‐OH‐T, 16__α__‐OH‐T, 16__β__‐OH‐T and 2__α__‐OH‐T, in __in vitro__ samples. The analytical method involves direct dilution of samples with acetonitrile containing an internal standard, followed by separation of testosterone and the four metabolites on an Acquity UPLCTM C~18~ column and detected by selected reaction monitoring (SRM) in positive ionization mode using turbo ionspray ionization. The parent compound and its metabolites investigated were well separated (Rs >1.5) with a run time of 4 min under a gradient condition. The method was partially validated. The linear concentration range was 0.01 to 5 μM for all the compounds of interest. Inter‐assay mean bias and relative standard deviation (RSD) were in the range of −12% to 8% and 4.1% to 8.5%, respectively. Intra‐assay mean bias and RSD were in the range of −8.0% to 5.2% and 3.4% to 9.6%, respectively. The lower limit of quantitation for this assay was 0.01 μM. The

Who reads Ultra-performance Liquid Chromatography/tandem Mass Spectrometric Determination of Testosterone and Its Metabolites in in Vitro Samples?

Author

Ganfeng Wang; Yunsheng Hsieh; Xiaoming Cui; Kuo-Chi Cheng; Walter A. Korfmacher

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2006

Language

EN

Field

Chemistry (Physical Sciences)