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Structural and functional characterization of a putative polysaccharide deacetylase of the human parasite Encephalitozoon cuniculi by Jonathan E. Urch; Ramon Hurtado-Guerrero; Damien Brosson; Zhanliang Liu; Vincent G. H. Eijsink; Catherine Texier; Daan M. F. van Aalten is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Structural and functional characterization of a putative polysaccharide deacetylase of the human parasite Encephalitozoon cuniculi about?
## Abstract The microsporidian __Encephalitozoon cuniculi__ is an intracellular eukaryotic parasite considered to be an emerging opportunistic human pathogen. The infectious stage of this parasite is a unicellular spore that is surrounded by a chitin containing endospore layer and an external proteinaceous exospore. A putative chitin deacetylase (ECU11\_0510) localizes to the interface between the plasma membrane and the endospore. Chitin deacetylases are family 4 carbohydrate esterases in the CAZY classification, and several bacterial members of this family are involved in evading lysis by host glycosidases, through partial de‐__N__‐acetylation of cell wall peptidoglycan. Similarly, ECU11\_0510 could be important for __E. cuniculi__ survival in the host, by protecting the chitin layer from hydrolysis by human chitinases. Here, we describe the biochemical, structural, and glycan binding properties of the protein. Enzymatic analyses showed that the putative deacetylase is unable to deacetylate chitooligosaccharides or crystalline β‐chitin. Furthermore, carbohydrate microarray analysis revealed that the protein bound neither chitooligosaccharides nor any of a wide range of other glyc
Who reads Structural and functional characterization of a putative polysaccharide deacetylase of the human parasite Encephalitozoon cuniculi?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jonathan E. Urch; Ramon Hurtado-Guerrero; Damien Brosson; Zhanliang Liu; Vincent G. H. Eijsink; Catherine Texier; Daan M. F. van Aalten
- Publisher
- Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)